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Yeast (Budding, Fission, and Candida)

World-Wide Web Virtual Library -- Biosciences
This section of the Virtual Library describes the yeast model organisms: Saccharomyces cerevisiae (budding, bakers', and sometimes brewers'), Schizosaccharomyces pombe (fission), and Candida albicans.

Contents of this page


Yeast information for the non-specialist

[X] Generally speaking:What are yeasts?
[X]Here are some pictures of yeast [X] [X] [X]...
[X] The Art of Brewing, courtesy of Miller Brewing Company.
[X] Information about yeast and bread baking, from Fleischmann's and Red Star Yeast companies.
[X] Information about the yeast-based foods Marmite and Vegemite.

[Buds] Saccharomyces cerevisiae information

Resources for educators
[X] Genetics Education Network. Tom Manney's (Kansas State University) yeast experiments for undergrads and high school students. A well-done and informative web site for yeast in the classroom.
[X] Research Link 2000. Aimed at undergraduate classrooms, this is an offshoot of Tom Manney's yeast experiments. It also lists other model organisms.
[X] Using Yeast As An Ultraviolet Light Measurement Tool. This is a Tom Manney protocol rewritten by Kevin Conant, a participant in the Woodrow Wilson Biology Institute 1993.
[x] MendelWeb, an educational resource on the origins of classical genetics.
[x] National Science Teachers Association. Resources for all science teachers from kindergarten to college.
[x] National Association of Biology Teachers. High school teachers predominate in this organization, but there are resources for post-secondary education as well.
[x] American Society for Microbiology. The ASM website lists resources in its Education section. The ASM publishes numerous books on microbiology education at various levels.
[x] Discovering Genomics, Proteomics, and Bioinformatics This website describes a course and the accompanying textbook written by A. Malcolm Campbell and Laurie J. Heyer.
[x] Genome Consortium for Active Teaching (GCAT) Description of the consortium and how it is working to bring functional genomics methods into the undergraduate biology curriculum.
[x] MicroArray Genome Imaging and Clustering Tool (MAGIC) Open source software for analysis of large-scale gene expression datasets; developed by Laurie Heyer and her undergraduate students at Davidson College, North Carolina.
General S. cerevisiae information
[X] An introduction to yeast, the most ideal eukaryotic microorganism for biological studies.
[X] An updated version the yeast primer Getting Started with Yeast written by Fred Sherman.
[X] Yeast Molecular Biology: A Short Compendium on Basic Features and Novel Aspects written by Horst Feldmann at the University of Munich
[X] Download a guide to S. cerevisiae nomenclature, published in Trends in Genetics.
[X] The Saccharomyces Genome Database (SGD) provides a wealth of yeast information. An introduction to SGD is found on the Getting Started with SGD page.
[X] A tutorial from the University of Saskatchewan provides an introduction to SGD and to S. cerevisiae molecular biology and genetics.
[X] SGD maintains a list of websites with relevance to S. cerevisiae molecular biology and genetics and to general molecular biology.
[X] Search the Yeast BioSci (a.k.a. BioNet) Electronic Conference
[X] Usenet group on yeast molecular biology: bionet.molbio.yeast
Colleagues
[X] Search for yeast colleagues at the Saccharomyces Genome Database.
[X] Yeast Laboratory Home Pages
[X] The World Taxonomist Database allows you to search for taxonomists, some of whom specialize in yeasts.
Laboratory manuals, textbooks, and references
[X] Methods in Yeast Genetics: A Cold Spring Harbor Laboratory Course Manual, a series of laboratory manuals published by CSHL Press.
[X] The Molecular and Cellular Biology of the Yeast Saccharomyces, a monograph series containing comprehensive reviews about the biology of the yeast Saccharomyces cerevisiae published by CSHL Press.
[X] NCBI Bookshelf, a growing collection of biomedical books that can be searched directly online.
Stock centers and strain collections
[X] American Type Culture Collection (ATCC) maintains yeast stocks and clones.
[X] EUROSCARF, the EUROpean Saccharomyces Cerevisiae ARchive for Functional analysis, maintains a collection of systematic deletion strains searchable by gene name.
[X] National Collection of Yeast Cultures (NCYC) maintains over 3,100 non-pathogenic yeasts, including type strains, strains of general interest for education and research, strains of industrial importance, and genetically marked strains.
[X] Yeast-GFP Clone Collection from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, consisting of C-terminal tagged open reading frames (ORFs). Hosted by Invitrogen.
[X] Yeast GST-fusion Collection developed in Eric Phizicky's laboratory. A total of 6080 individual yeast strains, each containing a plasmid expressing a different full-length yeast Open Reading Frame (ORF) fused to a glutathione S-transferase (GST). Hosted by ResGen Invitrogen.
[X] Yeast Knockout (YKO) Collection from the Saccharomyces Genome Deletion Consortium. Submit a comment to the Community Posting page. Read all comments.
[X] Yeast-TAP Fusion Library from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, containing open reading frames (ORFs) tagged with a high-affinity epitope and expressed from their natural chromosomal locations. Hosted by Open Biosystems.
[X] Yeast Tet-promoters Hughes Collection containing 800 essential yeast genes for which expression is regulated by doxycycline. Hosted by Open Biosystems.
[X] Yeast Transposon Insertion Library Collection, derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, from the Yale Genome Analysis Center.
Methods, protocols, and strains
[X] Commonly used lab strains, a table providing genotypes, references, and other notes for some of the most commonly used lab strains.
[X] Yeast Lab Protocols from the Botstein Lab, Stanford University.
[X] Yeast Lab Protocols from the Breeden Lab, Fred Hutchinson Cancer Research Center.
[X] Yeast Lab Protocols from the Gottschling Lab, Fred Hutchinson Cancer Research Center.
[X] The Definitive Yeast Transformation Homepage from the University of Manitoba.
[X] DNA Replication Protocols from the Fangman and Brewer Labs, University of Washington.
[X] Interaction Trap at Work information page from Erica Golemis's laboratory at the Fox Chase Cancer Center.
[X] The MGuide. Version 2.0, a complete guide to microarraying for the molecular biologist, from the Brown Lab, Stanford University.
[X] The NCRR Yeast Resource Center at the University of Washington facilitates the study of yeast protein complexes by providing access to techniques such as mass spectrometry, two-hybrid arrays, deconvolution fluorescence microscopy and protein structure prediction.
[X] Full text of the paper (Erdeniz et al. (1997) Genome Res. 7:1174-1183) describing PCR-Based Allele Replacement. Available to the public without password restriction and at no charge, through a special arrangement with Genome Research arranged by Rodney Rothstein.
[X] Find cloning vectors from NCBI's Taxonomy Browser.
[X] Tritech Research maintains a list of links to S. cerevisiae information and protocols, and sells reagents and equipment for S. cerevisiae research.
[X] VectorDB, collected sequences of cloning vectors for use in a variety of organisms.
[X] Yeast Cell Micromanipulation, a protocol for making a support rod with fiber optic needle attached.
[X] Yeast-GFP Clone Collection from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, consisting of C-terminal tagged open reading frames (ORFs). Hosted by Invitrogen.
[X] Yeast GST-fusion Collection developed in Eric Phizicky's laboratory. A total of 6080 individual yeast strains, each containing a plasmid expressing a different full-length yeast Open Reading Frame (ORF) fused to a glutathione S-transferase (GST). Hosted by ResGen Invitrogen.
[X] Yeast Knockout (YKO) Collection from the Saccharomyces Genome Deletion Consortium. Submit a comment to the Community Posting page. Read all comments.
[X] Yeast ProtoArrayTM Proteome Chips, containing 4,088 Saccharomyces cerevisiae open reading frames (ORFs) expressed as 5'-GST fusions from Invitrogen.
[X] Yeast-TAP Fusion Library from Dr. Erin O'Shea and Dr. Jonathan Weissman at UCSF, containing open reading frames (ORFs) tagged with a high-affinity epitope and expressed from their natural chromosomal locations. Hosted by Open Biosystems.
[X] Yeast Tet-promoters Hughes Collection containing 800 essential yeast genes for which expression is regulated by doxycycline. Hosted by Open Biosystems.
[X] Yeast Two-Hybrid Analisys at the University of Washington's Yeast Resource Center. The entire set of Saccharomyces cerevisiae open reading frames (ORFs) fused to the Gal4 activation domain is available as an array of ~6000 transformants.
[X] Yeast Transposon Insertion Library Collection, derived by using mini-transposons (mTns) to mutagenize a yeast genomic DNA library, from the Yale Genome Analysis Center.
[X] American Type Culture Collection (ATCC) maintains yeast stocks and clones.
[X] National Collection of Yeast Cultures (NCYC) maintains over 3,100 non-pathogenic yeasts, including type strains, strains of general interest for education and research, strains of industrial importance, and genetically marked strains.
[X] Common Access to Biological Resources and Information (CABRI) includes catalogs from European culture collections for yeast and other organisms.
Gene and protein sequence information
[X] Press release for the completion of the DNA Sequence in 1996
[X] European announcement of the complete Sequence, April 24, 1996.
[X] Yeast Genome Directory A collection of papers describing the sequencing of each chromosome of the S. cerevisiae genome
[X] Systematic Sequencing Table from SGD lists S. cerevisiae chromosome statistics, contacts and retrieval options
[X] Obtain files of S. cerevisiae gene and protein information via anonymous FTP from SGD.
[X] Saccharomyces Gene Registry at SGD.
[X] Yeast Genome Project Information from MIPS.
[X] Yeast Sequencing at the Sanger Centre.
[X] List of species specific documents from SWISS-PROT.
[X] Yeast Proteome Database (YPD), accessible only to subscribers.
[X] GeneQuiz - Consortium for large scale sequence analysis.
[X] Yeast Mitochondrial Protein Database from Bio-Molecular Engineering Center at Boston University.
[X] S. cerevisiae Codon Usage Tables.
Searching by yeast DNA and protein sequences
[X] BLAST Searching from SGD.
[X] FASTA Searching from SGD.
[X] Protein Search from MIPS.
Functional genomics resources
[X] SGD's Expression Connection allows you to search the results of multiple transcription profiling experiments.
[X] Yeast Cell Cycle Analysis Project web site accompanying Spellman et al. (1998) Mol Biol Cell 9:3273-3297
[X] Saccharomyces Genome Deletion Project from Stanford Biochemistry Department.
[X] Serial Analysis of Gene Expression (SAGE) at Johns Hopkins.
[X] TRIPLES, a database of transposon-insertion phenotypes, localization, and expression information.
[X] BIND, the Biomolecular Interaction Network Database, collects information about protein-protein interactions, complexes, and pathways.

[Fission] Schizosaccharomyces pombe information

[X] S. pombe genomic sequence published by V. Wood et al., Nature 415, 871-880 (2002).
[X] PomBase S. pombe database compiled at the Sanger Centre, UK.
[X] Blast Server for S. pombe compiled at the Sanger Centre, UK.
[X] European Schizosaccharomyces genome sequencing project.
[X] General information about S. pombe from the Forsburg Lab.
[X] S. pombe molecular genetics: plasmids, markers, maps and references from the Forsburg Lab.
[X] An S. pombe nomenclature guide: S. pombe genes mapped to S. cerevisiae genes from the Forsburg Lab.

[Candida] Candida albicans information

[X] Candida albicans pages at the NRC/BRI.
[X] Candida Genome Database at Stanford.
[X] Candida information from the Stanford Genome Technology Center.
[X] CandidaDB, a genomic database for C. albicans, part of the Galar Fungail Consortium project.
[X] Candida albicans information server from University of Minnesota.
[X] Candidiasis information at MEDLINE plus.
[X] Annotation of the C. albicans genome from the Agabian Lab.

Links to other fungal information

[X] General mycology information
[X] Fungal genome sequencing projects:
[X] Fungal genome database projects:
  • CryptoBase, a scientific database resource at UCSF for C. neoformans var. grubii (serotype A)

Biology Related Career Resources

Following is a list of sites that post biology related job openings. Please note that this is not a comprehensive list. Some of these sites may be free (may require registration) for job seekers, job posters or both.

[X] Open positions offered by Genetics Society of America
[X] Career Services offered by American Society for Cell Biology
[X]ASM Career Connections offered by American Society for Microbiology
[X] Career Resources offered by FASEB
[X] Job Openings offered by Society for Developmental Biology
[X] Science Careers and Science Next Wave offered by Science Magazine Online
[X] Nature Jobs offered by Nature Online
[X] Career Center offered by Biospace.com


S. cerevisiae and S. pombe images were provided by Frans Hochstenbach, NIH.
C. albicans image was provided by Stew Scherer, Univ. of Minnesota.

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Pages Updated On: Tuesday, Sep 13 21:22:54 PDT 2005
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